FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001617723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001617723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1429203
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACGTATATACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC54560.3817512277821975Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATACGTATATACGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTC52170.36502862084672366Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48470.33914006617674325No Hit
ACGTATATACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC42670.2985580075048821Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATACGTATATACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC38320.26812146350098626Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATACGTATATACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAG32750.22914869336266436Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCGGAT30590.21403537496072988TruSeq Adapter, Index 7 (97% over 35bp)
ATATACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCGGAT27080.18947623255758628TruSeq Adapter, Index 7 (97% over 35bp)
ATACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCGG21500.15043349335258882TruSeq Adapter, Index 7 (97% over 35bp)
ATATACGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCGG18800.1315418453501707TruSeq Adapter, Index 7 (97% over 35bp)
GTATATACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA14840.10383409494662409TruSeq Adapter, Index 7 (100% over 35bp)
ATACGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCGGAT14840.10383409494662409TruSeq Adapter, Index 7 (97% over 35bp)
GTATATACGTATATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC14730.10306443521319224Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)