FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001617724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001617724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1429203
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT74680.5225289899335503No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT43830.30667441923925437No Hit
ATACGTATATACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG43790.30639454297255186Illumina Single End PCR Primer 1 (100% over 32bp)
ATATACGTATATACGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG42700.29876791470490893Illumina Single End PCR Primer 1 (100% over 32bp)
ATATACGTATATACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG36910.25825582509972345Illumina Single End PCR Primer 1 (100% over 30bp)
ATATACGTATATACGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA32060.2243208277620464Illumina Single End PCR Primer 1 (100% over 34bp)
ACGTATATACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA30100.21060689069362434Illumina Single End PCR Primer 1 (100% over 34bp)
ATATACGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG24080.1684855125548995Illumina Single End PCR Primer 1 (100% over 42bp)
ACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG23530.16463721388774022Illumina Single End PCR Primer 1 (100% over 42bp)
ATATACGTATATACGTATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTT18860.13196165975022442No Hit
ATATACGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC18790.13147187628349508Illumina Single End PCR Primer 1 (100% over 40bp)
ATACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC16520.11558889814812871Illumina Single End PCR Primer 1 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)