FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001617725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001617725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2117259
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATATACGTATATACGTATATACGAGATCGGAAGAGCACACGTCTGAA142560.673323386510578Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATACGTATATACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAG121850.5755082396626959Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATACGTATATACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC69060.3261764384990216Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACAT65710.3103540946100595TruSeq Adapter, Index 2 (97% over 37bp)
ATATACGTATATACGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTC64970.3068590096913037Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACAT63530.30005776336291407TruSeq Adapter, Index 2 (97% over 37bp)
GTATATACGTATATACGTATATACAGATCGGAAGAGCACACGTCTGAACT57810.27304170155847723Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTATATACGTATATACGTATATACGTATATACGAGATCGGAAGAGCACA57110.2697355401488434No Hit
ACGTATATACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC50480.2384214685118826Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATACGTATATACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC49040.23162022218349293Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATAC48570.22940037095131016TruSeq Adapter, Index 2 (97% over 37bp)
ATATACGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATAC45970.21712034285838433TruSeq Adapter, Index 2 (97% over 37bp)
ATACGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACAT37980.17938287191127775TruSeq Adapter, Index 2 (97% over 37bp)
GTATATACGTATATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC34010.16063221363092564Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTATATACGTATATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC32540.15368927467069451Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAT31770.15005249712009724TruSeq Adapter, Index 2 (97% over 37bp)
GTATATACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT29740.14046462903215903TruSeq Adapter, Index 1 (97% over 35bp)
CGTATATACGTATATACGTATATACGTATATACGTATATACGTATATACG29220.13800862341357387No Hit
ATATACGTATATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT25430.12010812092427049TruSeq Adapter, Index 1 (97% over 35bp)
ATATACGTATATACGTATATACGTATATAGATCGGAAGAGCACACGTCTG21230.10027115246646724Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)