FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001617739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001617739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1307535
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATATATGTACATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAG45850.3506598293735923Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTACATATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTT13770.1053126684945336No Hit
ATATGTACATATATGTACATATAGATCGGAAGAGCACACGTCTGAACTCC13600.10401251209336652Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATACATATGTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCC13380.10232995675067971Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)