FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001617744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001617744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1289960
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG109800.8511891841607491Illumina Single End PCR Primer 1 (100% over 28bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT18450.1430276907811095No Hit
ATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATT17930.13899655803280722Illumina Single End PCR Primer 1 (96% over 33bp)
ACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATT16780.1300815529163695Illumina Single End PCR Primer 1 (96% over 33bp)
ATGTATACATATGTATACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG14230.11031349809296413Illumina Single End PCR Primer 1 (100% over 30bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)