FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001617747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001617747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1442051
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATATATGTACATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAG57380.3979054832318691Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATACATATGTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCC29030.2013104945664196Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATGTACATATATGTACATATAGATCGGAAGAGCACACGTCTGAACTCC17150.1189278326494694Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT16130.11185457379801408No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)