FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001617748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001617748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1442051
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATATATGTACATATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAG36270.25151676327674954Illumina Single End PCR Primer 1 (100% over 30bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT27950.1938211616648787No Hit
ATACATATGTATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG18550.12863622715146691Illumina Single End PCR Primer 1 (100% over 28bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)