FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001618305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001618305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences226812
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCC16540.7292383119058956Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7900.34830608609773733No Hit
ACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAG7570.3337565913620091TruSeq Adapter, Index 13 (97% over 38bp)
ATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAG6930.30553938945029363TruSeq Adapter, Index 13 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4340.19134790046382028No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT2480.1093416574078973No Hit
ATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAA2330.10272825070983897TruSeq Adapter, Index 13 (97% over 38bp)
GTATACATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAA2310.10184646315009789TruSeq Adapter, Index 13 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)