FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001618306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001618306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences226812
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG17540.773327689892951Illumina Single End PCR Primer 1 (100% over 28bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT13030.5744845951713313No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12950.5709574449323669No Hit
ATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGC7300.3218524593055041Illumina Single End PCR Primer 1 (96% over 33bp)
ACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGC6820.3006895578717176Illumina Single End PCR Primer 1 (96% over 33bp)
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT2740.12080489568453169No Hit
ATGTATACATATGTATACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG2540.11198702008712062Illumina Single End PCR Primer 1 (100% over 30bp)
ATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCG2270.10008288803061566Illumina Single End PCR Primer 1 (96% over 33bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)