FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001618307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001618307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3266105
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCC513731.5729133019299748Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGA113470.3474168772896156TruSeq Adapter, Index 6 (97% over 36bp)
ATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGA102040.3124210642340035TruSeq Adapter, Index 6 (97% over 36bp)
ATGTATACATATGTATACATAGATCGGAAGAGCACACGTCTGAACTCCAG63940.1957683540486298Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT40060.12265374199543493No Hit
ATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCG38920.11916334594264423TruSeq Adapter, Index 6 (97% over 36bp)
ATATGTATACATATGTATACATATAGATCGGAAGAGCACACGTCTGAACT33230.10174198318792568Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)