FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001618308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001618308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3266105
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG324620.993905584786772Illumina Single End PCR Primer 1 (100% over 28bp)
ATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTAT70830.21686381791154907Illumina Single End PCR Primer 1 (97% over 35bp)
ACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTAT62170.19034905491403367Illumina Single End PCR Primer 1 (97% over 35bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT45900.14053436738867855No Hit
ATGTATACATATGTATACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG42580.1303693543226565Illumina Single End PCR Primer 1 (100% over 30bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)