FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001618310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001618310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences881204
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG485935.514387133966709Illumina Single End PCR Primer 1 (100% over 28bp)
ATGTATACATATGTATACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG56150.6371963813146558Illumina Single End PCR Primer 1 (100% over 30bp)
ATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTA50360.5714908239181846Illumina Single End PCR Primer 1 (96% over 33bp)
ACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTA44800.508395331841435Illumina Single End PCR Primer 1 (96% over 33bp)
ATATGTATACATATGTATACATATAGATCGGAAGAGCGTCGTGTAGGGAA36100.4096667740954421Illumina Single End PCR Primer 1 (100% over 26bp)
ATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTC26640.30231365268428195Illumina Single End PCR Primer 1 (96% over 33bp)
GTATACATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTA20330.23070707804322266Illumina Single End PCR Primer 1 (96% over 33bp)
ATACATATGTATACATCTGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG19150.21731630814204203Illumina Single End PCR Primer 1 (100% over 28bp)
ATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTA18080.20517383035029346Illumina Single End PCR Primer 1 (96% over 33bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT12400.14071656506325436No Hit
CATATGTATACATATGTATACATATGAGATCGGAAGAGCGTCGTGTAGGG12250.1390143485503924Illumina Single End PCR Primer 1 (100% over 24bp)
ATACATATGTATAAATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG11470.1301628226835103Illumina Single End PCR Primer 1 (100% over 28bp)
ATACATATGTATCCATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG10560.11983604250548113Illumina Single End PCR Primer 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)