FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001618313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001618313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1328416
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCC572954.31303146002457Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGA92380.6954146893744129TruSeq Adapter, Index 6 (97% over 36bp)
ATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGA82920.6242020571869054TruSeq Adapter, Index 6 (97% over 36bp)
ATGTATACATATGTATACATAGATCGGAAGAGCACACGTCTGAACTCCAG64800.48779900272203885Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCG37790.2844741406306458TruSeq Adapter, Index 6 (97% over 36bp)
ATATGTATACATATGTATACATATAGATCGGAAGAGCACACGTCTGAACT36280.2731072194252403Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGA33980.255793365933563TruSeq Adapter, Index 6 (97% over 36bp)
GTATACATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGA29720.22372509816202152TruSeq Adapter, Index 6 (97% over 36bp)
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT18620.1401669356587093No Hit
CATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGG18480.13911304892443332TruSeq Adapter, Index 6 (97% over 36bp)
GTATACATATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGG16230.12217558355214028TruSeq Adapter, Index 6 (97% over 36bp)
CATATGTATACATATGTATACATATGAGATCGGAAGAGCACACGTCTGAA14360.10809866788716789Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)