FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001618314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001618314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1328416
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG359962.709693349071375Illumina Single End PCR Primer 1 (100% over 28bp)
ATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAG58290.43879326957820447Illumina Single End PCR Primer 1 (97% over 35bp)
ACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAG51640.38873364970009394Illumina Single End PCR Primer 1 (97% over 35bp)
ATGTATACATATGTATACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG43250.3255757232674102Illumina Single End PCR Primer 1 (100% over 30bp)
ATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGAT24460.1841290680027943Illumina Single End PCR Primer 1 (97% over 35bp)
ATATGTATACATATGTATACATATAGATCGGAAGAGCGTCGTGTAGGGAA23920.18006407631344398Illumina Single End PCR Primer 1 (100% over 26bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT21940.16515910678582613No Hit
GTATACATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAG21550.16222327945462867Illumina Single End PCR Primer 1 (97% over 35bp)
ATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAG18510.13933888179606388Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)