FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001618318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001618318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1300870
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG175081.3458685341348482Illumina Single End PCR Primer 1 (100% over 28bp)
ATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGC55100.4235626926595279Illumina Single End PCR Primer 1 (96% over 33bp)
ACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGC49190.37813155811111027Illumina Single End PCR Primer 1 (96% over 33bp)
ATGTATACATATGTATACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG23980.18433817368376548Illumina Single End PCR Primer 1 (100% over 30bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT21670.16658082667753119No Hit
ATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGA18090.13906078239947112Illumina Single End PCR Primer 1 (96% over 33bp)
GTATACATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGC17840.1371389915979306Illumina Single End PCR Primer 1 (96% over 33bp)
ATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGC16220.12468578720394813Illumina Single End PCR Primer 1 (96% over 33bp)
ATATGTATACATATGTATACATATAGATCGGAAGAGCGTCGTGTAGGGAA14050.10800464304657652Illumina Single End PCR Primer 1 (100% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)