FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001618328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001618328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1807981
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG230681.27589836397617Illumina Single End PCR Primer 1 (100% over 28bp)
ATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTA53630.2966292234265736Illumina Single End PCR Primer 1 (96% over 33bp)
ACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTA48290.2670935148101667Illumina Single End PCR Primer 1 (96% over 33bp)
ATGTATACATATGTATACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG29560.16349729338969823Illumina Single End PCR Primer 1 (100% over 30bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT27530.15226929929020272No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)