FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001618329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001618329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1305665
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCC475123.6389119720602143Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGA120600.9236672500220194TruSeq Adapter, Index 6 (97% over 36bp)
ATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGA110070.8430186916245745TruSeq Adapter, Index 6 (97% over 36bp)
ATGTATACATATGTATACATAGATCGGAAGAGCACACGTCTGAACTCCAG61740.4728624876978398Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCG46810.3585146266461918TruSeq Adapter, Index 6 (97% over 36bp)
ATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGA46390.3552978750291997TruSeq Adapter, Index 6 (97% over 36bp)
GTATACATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGA41430.3173095702190072TruSeq Adapter, Index 6 (97% over 36bp)
ATATGTATACATATGTATACATATAGATCGGAAGAGCACACGTCTGAACT38470.29463913025163424Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGG23860.18274212757483735TruSeq Adapter, Index 6 (97% over 36bp)
GTATACATATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGG20310.15555291747883263TruSeq Adapter, Index 6 (97% over 36bp)
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT17040.13050820846082264No Hit
CATATGTATACATATGTATACATATGAGATCGGAAGAGCACACGTCTGAA14590.11174382402836869Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCG13510.10347217701324612TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)