FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001618330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001618330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1305665
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG296582.271486177541713Illumina Single End PCR Primer 1 (100% over 28bp)
ATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCC68240.5226455484370033Illumina Single End PCR Primer 1 (96% over 33bp)
ACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCC62090.4755431140453332Illumina Single End PCR Primer 1 (96% over 33bp)
ATGTATACATATGTATACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG40910.31332692535987405Illumina Single End PCR Primer 1 (100% over 30bp)
ATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCG29110.22295152278723868Illumina Single End PCR Primer 1 (96% over 33bp)
GTATACATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCC26810.2053359782179962Illumina Single End PCR Primer 1 (96% over 33bp)
ATATGTATACATATGTATACATATAGATCGGAAGAGCGTCGTGTAGGGAA25960.1988258856597979Illumina Single End PCR Primer 1 (100% over 26bp)
ATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCC22640.17339823002071741Illumina Single End PCR Primer 1 (96% over 33bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT20400.1562422213967595No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)