FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001618331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001618331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1339088
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCC206391.5412728663090103Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGA61430.4587450563368502TruSeq Adapter, Index 6 (97% over 36bp)
ATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGA58330.4355949721004146TruSeq Adapter, Index 6 (97% over 36bp)
ATGTATACATATGTATACATAGATCGGAAGAGCACACGTCTGAACTCCAG29460.22000047793722294Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT25910.19348989760194998No Hit
ATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCG21390.15973558123140524TruSeq Adapter, Index 6 (97% over 36bp)
ATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGA20920.15622572975039728TruSeq Adapter, Index 6 (97% over 36bp)
GTATACATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGA19600.14636827452714085TruSeq Adapter, Index 6 (97% over 36bp)
ATATGTATACATATGTATACATATAGATCGGAAGAGCACACGTCTGAACT16070.12000704957403845Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGG14320.10693845363411517TruSeq Adapter, Index 6 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)