FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001618773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001618773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2537183
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT379671.4964233955532573No Hit
GTCCCGAGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCGGGTCGG316701.2482347548442505No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221540.8731731215288767No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121230.47781338594811645No Hit
CTATAATACCATGCTAATATATTCAAAGCTAAAAAGCTTGTTTTGAACAC107320.42298880293616975No Hit
GGCATAGACAGCCAGGATGTTGGCTTAGAAGCAGCCATCATTTAAAGAAA94340.3718297024692346No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90220.35559122065692544No Hit
GTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGG75880.29907184464029596No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73970.29154381059624No Hit
GTCCCGAGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGG64700.2550072265185444No Hit
GGCTAAAACAGTCAGGATGTTGGCTTAGAAGCAGCCATCATTTAAAGAAA61900.2439713650927032No Hit
GTATAAGACCGTACCGCAAACCGACACAGGTGGGTAGGATGAGAATTCTA58800.23175308994266478No Hit
CCCTAGTAGTGTGATGAGAACATCTGAGCACGAATGGGTTGCAAAAAATT58750.23155602098863187No Hit
GCAAAAAATTGGTGGCTGCGACTGTTTAATAAAAACACAGCACTCTGCAA56880.22418564210780223No Hit
GTCTTTCCGCGGGGAGATTGCATCATCACAAACATTTCAACTTCGCTGAG48810.1923787129268957No Hit
GTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCGGGTCGG48720.1920239888096365No Hit
TTATCGTTACGGCCGCCGTTTACTGGGACTTCAATCAAGAGCTTGCACCC47970.18906795449914335No Hit
GTCTTATACAACTGAAGCTTAGTGGCTTTTCTTGGAAGCTTGGTATCAAT46720.18414123064832139No Hit
TTATTGTTACGGCCGCCGTTTACTGGGACTTCAATCAAGAGCTTGCACCC46080.18161874803670056No Hit
GACCATGGGTGCTAACGTCCGTGGTCAAGAGGGAAACAACCCAGACCGAC45730.1802392653584704No Hit
AACCCGGGAGAAGGAACTCGGCAAATTAGCACCGTAACTTCGGGAGAAGG45340.17870212751701395No Hit
AATTAGCACCGTAACTTCGGGAGAAGGTGCGCCCTGGTAGTGTGTATAGA45080.17767736895604297No Hit
GTTATGACTAGGGGGATCATAAGATCTTACCAACTCATTGCAAACTCCGA42380.16703564543826757No Hit
GCAAAAAATTGGTGGCTGCGACTGTTTATTAAAAACACAGCACTCTGCAA41150.16218774916905876No Hit
TTATAGTTACGGCCGCCGTTTACTGGGACTTCAATCAAGAGCTTGCACCC40530.15974409413905105No Hit
CCTTTATACAATGCGATGTGGGGACGGAGAAGGTTAGGTCAGCCGGGTGT38680.1524525428398346No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38660.15237371525822144No Hit
GTATTATAGAATATGACGAATTCAGGTCATATAAAGTTCTGGGGGCCGCA36850.14523981912223122No Hit
CTACCAGGGCGCACCTTCTCCCGAAGTTACGGTGCTAATTTGCCGAGTTC36760.14488509500497204No Hit
ATATTAACCTGTTTCCCATCAGCTACGCATCTCTGCCTCACCTTAGGGGC35910.1415349227864131No Hit
GTACTAGTTCACTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATGGT35640.14047075043463558No Hit
GCGCGAGATGAGTATTCTAAGGCGCTTGAGAGAACCCGGGAGAAGGAACT35500.1399189573633435No Hit
GGTCAATATTCCTGTACCGATTACAAGTGCGATGTGGGGACGGATCTTAA35460.1397613022001172No Hit
GTATTTGGGATGAGTACAACCTAAAACCATTAACGAGCAACAATAAGAGG35450.13972188840931066No Hit
GAGCACCGGGTGCTAACGTCCGGACTCAAGAGGGAAACAACCCAGACCGC34960.13779061265978845No Hit
CGATAGGGTTGCAGTGAAAAGGTGGCTGCGACTGTTTAATAAAAACACAG34020.13408571632397032No Hit
GATCTTAATAGGTTATCCGGCTGTTGGATGTGCCGGTTTAGACAGATGTA33280.13116909580428374No Hit
CTACCAGGGCGCACCTTCTTCCGAAGTTACGGTGTCAATTTGCCGAGTTC31150.12277395836248313No Hit
ACCTTTATACAATGCGATGTGGGGACGGAGAAGGTTAGGTCAGCCGGGTG31090.12253747561764365No Hit
AACCTTCTCCGTCCCCACATCGCATTGTATAAAGGTACAGGAATATTAAC30910.1218280273831253No Hit
GCGTCTAGTTGCTGGGTGTAGACCCGAAACCAAGTGATCTATCCATGGCC29520.1163495104610113No Hit
ACCTAAAACCATTAACGAGCAACAATAAGAGGGCAAGTCTGGTGCCAGCA29430.11599478634375211No Hit
AGTCATAACAGTGCTCTACCCCCGAGAATGATATACGAGGCACTACCTAA29160.11493061399197456No Hit
CGATAGGGTTGCAGTGAAAAGGTGGCTGCGACTGTTTATTAAAAACACAG29010.11433940712987592No Hit
GCGCGAGATGAGTATTCTAAGGCGCTTGAGAGAACTCAGGAGAAGGAACT28680.11303875203325893No Hit
AACTAGCGGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAA28250.11134395902857618No Hit
AACCCGGGAGAAGGAACTCGGCAAATTGACACCGTAACTTCGGAAGAAGG28090.11071333837567097No Hit
TCCCAAATACTGTTCCAATTTGGACCCAGCTCTGGTATTTTTCGTCACTA28070.11063451079405782No Hit
GTCGATTACGAGTATTTAGCCTTGGAGGATGGTCCCCCCATGTTCAGACA27940.11012213151357234No Hit
GTATAAAGGTACAGGAATATTAACCTGTTTCCCATCAGCTACGCATCTCT27800.10957033844228029No Hit
GCCAGTTACCGAAGCTGCGGATTTGCAATTTATTGCAAGTGGTAGGAGAG27360.10783613164679096No Hit
CGGGTAGATTGCATCTTCACAAACATTTCAACTTCGCTGAGTCCCGAGAG26920.10610192485130163No Hit
ATATTAACCTGTTTCCCATCAGCTACGCATCTCTGCCTCGCCTTAGGGGC26830.10574720073404244No Hit
AATATATTCAAAGCTAAAAAGCTTGTTTTGAACACTCTAATTTATTCAAA26770.10551071798920299No Hit
TCAATCAAGAGCTTGCACCCCATCATTTAATCTTCCAGCACCGGGCAGGC26770.10551071798920299No Hit
GTTTATTAAAAACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGT26360.10389475256613338No Hit
ACACACGACCGTACCGCAAACCGACACTGGTGCGCGAGATGAGTATTCTA26010.10251526988790324No Hit
ACCTTTATACAATGCGATGTGGGGACGGATCTTAATAGGTTATCCGGCTG25960.10231820093387035No Hit
GTAATCGGTACAGGAATATTAACCTGTTTCCCATCAGCTACGCATCTCTG25720.10137226995451255No Hit
ATCATTTAATCTTCCAGCACCGGGCAGGCGTCACACCCTATACGTCCACT25560.10074164930160734No Hit
GTCCCGAGAGGAGACAGTGTGGCCATCATTACGCCATTCGTGCAGGTCGG25540.10066282171999419No Hit
CTCCTGAGTTCTCTCAAGCGCCTTAGAATACTCATCTCGCGCACCAGTGT25480.10042633897515472No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)