FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001618774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001618774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2537183
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCGAGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCGGGTCGG229620.9050194645005899No Hit
CTATAATACCATGCTAATATATTCAAAGCTAAAAAGCTTGTTTTGAACAC94260.371514392142782No Hit
GGCATAGACAGCCAGGATGTTGGCTTAGAAGCAGCCATCATTTAAAGAAA74360.29308094843769644No Hit
GTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGG66980.2639935708224436No Hit
CCCTAGTAGTGTGATGAGAACATCTGAGCACGAATGGGTTGCAAAAAATT55190.21752471146149094No Hit
GGCTAAAACAGTCAGGATGTTGGCTTAGAAGCAGCCATCATTTAAAGAAA51370.20246864337337905No Hit
GCAAAAAATTGGTGGCTGCGACTGTTTAATAAAAACACAGCACTCTGCAA51040.20116798827676205No Hit
GTATAAGACCGTACCGCAAACCGACACAGGTGGGTAGGATGAGAATTCTA50140.19762074710417024No Hit
GTCCCGAGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGG49010.1931669887430272No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46950.1850477478368726No Hit
GTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCGGGTCGG44390.17495781739038926No Hit
GTCTTTCCGCGGGGAGATTGCATCATCACAAACATTTCAACTTCGCTGAG42870.16896692118778975No Hit
TTATCGTTACGGCCGCCGTTTACTGGGACTTCAATCAAGAGCTTGCACCC42860.16892750739698317No Hit
GTCTTATACAACTGAAGCTTAGTGGCTTTTCTTGGAAGCTTGGTATCAAT40650.16021705962872998No Hit
GACCATGGGTGCTAACGTCCGTGGTCAAGAGGGAAACAACCCAGACCGAC40400.1592317148585656No Hit
TTATTGTTACGGCCGCCGTTTACTGGGACTTCAATCAAGAGCTTGCACCC39580.15599978401242637No Hit
GTTATGACTAGGGGGATCATAAGATCTTACCAACTCATTGCAAACTCCGA38840.1530831634927398No Hit
GCAAAAAATTGGTGGCTGCGACTGTTTATTAAAAACACAGCACTCTGCAA38120.15024537055466633No Hit
AATTAGCACCGTAACTTCGGGAGAAGGTGCGCCCTGGTAGTGTGTATAGA37380.14732875003497975No Hit
AACCCGGGAGAAGGAACTCGGCAAATTAGCACCGTAACTTCGGGAGAAGG37140.14638281905562192No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36300.1430720606278696No Hit
TTATAGTTACGGCCGCCGTTTACTGGGACTTCAATCAAGAGCTTGCACCC34890.1375147161241424No Hit
GTACTAGTTCACTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATGGT34240.134952819721715No Hit
ATATTAACCTGTTTCCCATCAGCTACGCATCTCTGCCTCACCTTAGGGGC32850.12947430279960098No Hit
CCTTTATACAATGCGATGTGGGGACGGAGAAGGTTAGGTCAGCCGGGTGT32580.12841013044782343No Hit
CTACCAGGGCGCACCTTCTCCCGAAGTTACGGTGCTAATTTGCCGAGTTC32250.12710947535120642No Hit
GGTCAATATTCCTGTACCGATTACAAGTGCGATGTGGGGACGGATCTTAA31970.12600588920862232No Hit
GATCTTAATAGGTTATCCGGCTGTTGGATGTGCCGGTTTAGACAGATGTA30370.11969968267957021No Hit
GAGCACCGGGTGCTAACGTCCGGACTCAAGAGGGAAACAACCCAGACCGC30180.11895082065424528No Hit
GTATTTGGGATGAGTACAACCTAAAACCATTAACGAGCAACAATAAGAGG30170.1189114068634387No Hit
CGATAGGGTTGCAGTGAAAAGGTGGCTGCGACTGTTTAATAAAAACACAG29990.11820195862892034No Hit
TCAATCAAGAGCTTGCACCCCATCATTTAATCTTCCAGCACCGGGCAGGC29460.11611302771617182No Hit
GTATTATAGAATATGACGAATTCAGGTCATATAAAGTTCTGGGGGCCGCA28910.11394526922181017No Hit
GCGTCTAGTTGCTGGGTGTAGACCCGAAACCAAGTGATCTATCCATGGCC27560.10862440746292246No Hit
GTCGATTACGAGTATTTAGCCTTGGAGGATGGTCCCCCCATGTTCAGACA26550.10464361459145831No Hit
GCGCGAGATGAGTATTCTAAGGCGCTTGAGAGAACCCGGGAGAAGGAACT26550.10464361459145831No Hit
AACTAGCGGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAA26250.10346120086726104No Hit
GTTTATTAAAAACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGT25790.10164816649015856No Hit
CGATAGGGTTGCAGTGAAAAGGTGGCTGCGACTGTTTATTAAAAACACAG25500.10050516655676787No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)