FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001618889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001618889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences978615
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCC176151.7999928470338182Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCG64590.6600144081175947TruSeq Adapter, Index 6 (97% over 36bp)
ATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCG52330.5347353147049657TruSeq Adapter, Index 6 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38370.39208473199368493No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27480.2808050152511457No Hit
ATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAA20420.20866224204615708TruSeq Adapter, Index 6 (97% over 36bp)
GTATACATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAA19360.19783060754229193TruSeq Adapter, Index 6 (97% over 36bp)
CATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGC16000.16349636986966273TruSeq Adapter, Index 6 (97% over 36bp)
ATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCG15350.15685432984370767TruSeq Adapter, Index 6 (97% over 36bp)
ATGTATACATATGTATACATAGATCGGAAGAGCACACGTCTGAACTCCAG14660.14980354889307848Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACATATATGTACATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAG14590.14908825227489872Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTATACATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCG14170.14479647256582007TruSeq Adapter, Index 6 (97% over 36bp)
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT14160.14469428733465153No Hit
GTATACATATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGC13580.13876754392687626TruSeq Adapter, Index 6 (97% over 36bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT11170.1141409032152583No Hit
ATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCG10610.1084185302698201TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)