FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001618890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001618890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences978615
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG184331.883580366129683Illumina Single End PCR Primer 1 (100% over 28bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT77920.7962273212652575No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61240.6257823556761342No Hit
ATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTA59190.6048343832865836Illumina Single End PCR Primer 1 (96% over 33bp)
ACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTA49020.5009120031881792Illumina Single End PCR Primer 1 (96% over 33bp)
GTATACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACG19690.2012027201708537Illumina Single End PCR Primer 1 (96% over 33bp)
ATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACG18280.18679460257608968Illumina Single End PCR Primer 1 (96% over 33bp)
ATGTATACATATGTATACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG17110.17483893052937058Illumina Single End PCR Primer 1 (100% over 30bp)
ACATATATGTACATATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAG14880.15205162397878633Illumina Single End PCR Primer 1 (100% over 30bp)
GTATACATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTA14390.1470445476515279Illumina Single End PCR Primer 1 (96% over 33bp)
GTATACATATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGT14250.14561395441516836Illumina Single End PCR Primer 1 (96% over 33bp)
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT13460.13754132115285378No Hit
ATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTA12950.13232987436325827Illumina Single End PCR Primer 1 (96% over 33bp)
CATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGT12190.1245637967944493Illumina Single End PCR Primer 1 (96% over 33bp)
ATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAG10230.1045354914854156Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)