FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001618909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001618909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1502344
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCC655004.359853668667096Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCA154321.027194836868254TruSeq Adapter, Index 2 (97% over 37bp)
ATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCA142710.9499155985579867TruSeq Adapter, Index 2 (97% over 37bp)
ATGTATACATATGTATACATAGATCGGAAGAGCACACGTCTGAACTCCAG95490.6356067585053756Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCT68990.45921573221579076TruSeq Adapter, Index 2 (97% over 37bp)
ATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCA59620.39684652782585084TruSeq Adapter, Index 2 (97% over 37bp)
ATATGTATACATATGTATACATATAGATCGGAAGAGCACACGTCTGAACT59050.393052456694339Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTATACATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCA54990.366028020213746TruSeq Adapter, Index 2 (97% over 37bp)
CATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTC29880.1988892024729356TruSeq Adapter, Index 2 (97% over 37bp)
CATATGTATACATATGTATACATATGAGATCGGAAGAGCACACGTCTGAA28130.18724073847268002Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTATACATATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTC24970.16620694062078992TruSeq Adapter, Index 2 (97% over 37bp)
ACATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCT23210.15449191396910428TruSeq Adapter, Index 2 (97% over 37bp)
GTATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCT21710.14450751625459948TruSeq Adapter, Index 2 (97% over 37bp)
GTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG17810.11854808219688699TruSeq Adapter, Index 2 (100% over 35bp)
ATACATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG17190.11442119780822502TruSeq Adapter, Index 2 (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)