FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001618910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001618910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1502344
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG417452.778657883946686Illumina Single End PCR Primer 1 (100% over 28bp)
ATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTAT96660.6433945887226894Illumina Single End PCR Primer 1 (97% over 35bp)
ACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTAT87570.5828891385727902Illumina Single End PCR Primer 1 (97% over 35bp)
ATGTATACATATGTATACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG62410.4154175075748297Illumina Single End PCR Primer 1 (100% over 30bp)
ATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCT44610.29693598802937277Illumina Single End PCR Primer 1 (97% over 35bp)
ATATGTATACATATGTATACATATAGATCGGAAGAGCGTCGTGTAGGGAA39870.2653852912515376Illumina Single End PCR Primer 1 (100% over 26bp)
GTATACATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTAT37130.24714712475970885Illumina Single End PCR Primer 1 (97% over 35bp)
ATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTAT34690.23090583781078103Illumina Single End PCR Primer 1 (97% over 35bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT25030.1666063165293701No Hit
GTATACATATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTA17270.11495369901966528Illumina Single End PCR Primer 1 (97% over 35bp)
CATATGTATACATATGTATACATATGAGATCGGAAGAGCGTCGTGTAGGG17000.1131565074310544Illumina Single End PCR Primer 1 (100% over 24bp)
CATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTA15370.10230679524795919Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)