FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001618911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001618911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568276
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCC172111.0974471330301554Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGTATACATATGTATACATAGATCGGAAGAGCACACGTCTGAACTCCAG31030.19786058066309756Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCA30160.19231308774730976TruSeq Adapter, Index 2 (97% over 37bp)
ATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCA27640.17624448757744174TruSeq Adapter, Index 2 (97% over 37bp)
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT20120.12829374421339101No Hit
ATATGTATACATATGTATACATATAGATCGGAAGAGCACACGTCTGAACT18680.11911168697346641Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)