FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001618912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001618912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568276
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG105580.673223335688361Illumina Single End PCR Primer 1 (100% over 28bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT29350.1871481805498522No Hit
ATGTATACATATGTATACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG20050.12784739420867247Illumina Single End PCR Primer 1 (100% over 30bp)
ATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTA18330.11687993694987361Illumina Single End PCR Primer 1 (96% over 33bp)
ACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTA16110.10272426537165653Illumina Single End PCR Primer 1 (96% over 33bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)