FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001618913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001618913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1257823
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCC96350.7660060278751462Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGC43530.3460741296668927TruSeq Adapter, Index 8 (97% over 36bp)
ATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGC38340.30481236231170844TruSeq Adapter, Index 8 (97% over 36bp)
ATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGC16300.1295889803255307TruSeq Adapter, Index 8 (97% over 36bp)
ATGTATACATATGTATACATAGATCGGAAGAGCACACGTCTGAACTCCAG16000.1272039070680056Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTATACATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGC15770.125375350903903TruSeq Adapter, Index 8 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)