FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001619327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001619327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1103997
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80660.7306179274037882No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53660.4860520454312829No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT25120.2275368501907161No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT22650.20516360098804617No Hit
ACATATATGTACATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAG21920.19855126417915991Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16770.15190258669181164No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16410.1486417082655116No Hit
ATACATATGTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCC15430.1397648725494725Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT15150.13722863377346134No Hit
AGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14550.13179383639629455No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13470.12201120111739434No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11680.1057973889421801No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)