FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001619328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001619328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1103997
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT234202.121382576220769No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA138091.2508186163549357No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT30110.27273624837748656No Hit
ACATATATGTACATATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAG22460.20344258181861002Illumina Single End PCR Primer 1 (100% over 30bp)
ATACATATGTATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG16310.14773590870265046Illumina Single End PCR Primer 1 (100% over 28bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15680.14202937145662534No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14480.13115977670229176No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11580.10489158937931897No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)