FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001619329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001619329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857558
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCC47720.5564638193568249Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT36890.4301749852488112TruSeq Adapter, Index 27 (97% over 39bp)
ATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT35230.410817693963557TruSeq Adapter, Index 27 (97% over 39bp)
ATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT11840.1380664631430177TruSeq Adapter, Index 27 (97% over 39bp)
GTATACATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT10910.12722171561573678TruSeq Adapter, Index 27 (97% over 39bp)
ATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCG10730.12512273222335982TruSeq Adapter, Index 27 (97% over 39bp)
GTATACATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCG9990.11649357827692121TruSeq Adapter, Index 27 (97% over 39bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)