FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001619330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001619330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857558
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG49980.5828177219500021Illumina Single End PCR Primer 1 (100% over 28bp)
ATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTAT36570.42644345921791876Illumina Single End PCR Primer 1 (97% over 35bp)
ACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTAT35500.41396616905212247Illumina Single End PCR Primer 1 (97% over 35bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT31360.3656895510274524No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT12040.14039866691232547No Hit
GTATACATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTAT11980.13969900578153313Illumina Single End PCR Primer 1 (97% over 35bp)
ATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTAT10980.1280379869349945Illumina Single End PCR Primer 1 (97% over 35bp)
GTATACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAG10830.12628883410801367Illumina Single End PCR Primer 1 (97% over 35bp)
ATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAG10150.11835934129236739Illumina Single End PCR Primer 1 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)