FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001619333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001619333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836785
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA125991.5056436241089408No Hit
ACATATATGTACATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAG125311.497517283412107Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73220.8750156850325951No Hit
ATATGTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGC36610.43750784251629754TruSeq Adapter, Index 8 (97% over 36bp)
CATATATGTACATATATGTACATATATGAGATCGGAAGAGCACACGTCTG36230.4329666521268904Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATGTACATATATGTACATATAGATCGGAAGAGCACACGTCTGAACTCC34600.413487335456539Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTACATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGC33370.39878821919608975TruSeq Adapter, Index 8 (97% over 36bp)
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT31210.37297513698261797No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT30530.36484879628578426No Hit
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23260.27796865383581204No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21630.2584893371654607No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21490.2568162670219949No Hit
ATATGTACATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGC21270.2541871567965487TruSeq Adapter, Index 8 (97% over 36bp)
ATGTACATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGC20520.2452242810279821TruSeq Adapter, Index 8 (97% over 36bp)
ATATATACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGC20390.24367071589476386TruSeq Adapter, Index 8 (97% over 36bp)
ACGTATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGC19900.2378149703926337TruSeq Adapter, Index 8 (97% over 36bp)
ATATATGTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGC19120.22849357959332442TruSeq Adapter, Index 8 (97% over 36bp)
AGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17680.21128485811767656No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16690.19945386210316868No Hit
ATATACGTATATATACGTATATAGATCGGAAGAGCACACGTCTGAACTCC16640.1988563370519309Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTACATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGC16050.19180554144732517TruSeq Adapter, Index 8 (97% over 36bp)
GTATATATACGTATATATACAGATCGGAAGAGCACACGTCTGAACTCCAG15780.18857890617064121Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CATATATGTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCG14070.16814354941830936TruSeq Adapter, Index 8 (97% over 36bp)
CATATATGTACATATATGTACATATATGTACATATATGAGATCGGAAGAG13510.16145126884444633No Hit
CGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13160.1572685934857819No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13140.15702958346528678No Hit
CGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12830.1533249281476126No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT12130.14495957743028376No Hit
CGTATATACGTATATACGTATATACGAGATCGGAAGAGCACACGTCTGAA12070.14424254736879843Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11990.143286507286818No Hit
GTACATATATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCG11950.1428084872458278TruSeq Adapter, Index 8 (97% over 36bp)
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11840.14149393213310468No Hit
ACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGC11400.13623571168221227TruSeq Adapter, Index 8 (97% over 36bp)
ATATACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGC11370.1358771966514696TruSeq Adapter, Index 8 (97% over 36bp)
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10910.1303799661800821No Hit
CGTATATACGTATATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC10170.12153659542176307Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10050.12010253529879239No Hit
ATACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGC9740.11639787998111821TruSeq Adapter, Index 8 (97% over 36bp)
GGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9520.11376876975567202No Hit
ATGTACATATATGTACATAGATCGGAAGAGCACACGTCTGAACTCCAGTC9250.11054213447898803Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT8940.10683747916131384No Hit
ATATACGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGC8930.10671797415106629TruSeq Adapter, Index 8 (97% over 36bp)
GCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8650.10337183386413475No Hit
ACATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGC8500.10157925871042144TruSeq Adapter, Index 8 (97% over 36bp)
GGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8480.10134024868992632No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8450.10098173365918366No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)