FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001619334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001619334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836785
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT286203.420233393285013No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA231492.766421482220642No Hit
ACATATATGTACATATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAG126261.5088702593856247Illumina Single End PCR Primer 1 (100% over 30bp)
ATATGTACATATATGTACATATAGATCGGAAGAGCGTCGTGTAGGGAAAG43410.5187712494846347Illumina Single End PCR Primer 1 (100% over 28bp)
CATATATGTACATATATGTACATATATGAGATCGGAAGAGCGTCGTGTAG38630.46164785458630353Illumina Single End PCR Primer 1 (100% over 22bp)
GTACATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAG36240.433086157137138Illumina Single End PCR Primer 1 (97% over 35bp)
ATATGTACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAG33190.3966371290116338Illumina Single End PCR Primer 1 (97% over 35bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28300.3381991790005796No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT26920.32170748758641704No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25200.30115262582383767No Hit
ATGTACATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTAT21310.2546651768375389Illumina Single End PCR Primer 1 (97% over 35bp)
ATATGTACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTAT20440.24426824094600166Illumina Single End PCR Primer 1 (97% over 35bp)
ACGTATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTAT20280.2423561607820408Illumina Single End PCR Primer 1 (97% over 35bp)
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19950.23841249544387147No Hit
ATATATACGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTAT19610.2343493250954546Illumina Single End PCR Primer 1 (97% over 35bp)
GTACATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTAT19060.2277765495318391Illumina Single End PCR Primer 1 (97% over 35bp)
AAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18480.22084525893748094No Hit
ATATACGTATATATACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAG18120.216543078568569Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATGTACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTAT16020.1914470264165825Illumina Single End PCR Primer 1 (97% over 35bp)
GTATATATACGTATATATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAG15990.19108851138583985Illumina Single End PCR Primer 1 (100% over 30bp)
CGTATATACGTATATACGTATATACGAGATCGGAAGAGCGTCGTGTAGGG15820.18905692621163142Illumina Single End PCR Primer 1 (100% over 24bp)
CATATATGTACATATATGTACATATATGTACATATATGAGATCGGAAGAG15130.18081108050455016No Hit
AAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13940.16658998428509114No Hit
GTACATATATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTA13910.16623146925434848Illumina Single End PCR Primer 1 (97% over 35bp)
AAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13550.1619292888854365No Hit
CATATATGTACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTA12020.14364502231756066Illumina Single End PCR Primer 1 (97% over 35bp)
ATGTACATATATGTACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG11850.14161343714335223Illumina Single End PCR Primer 1 (100% over 32bp)
ACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAG11380.13599670166171718Illumina Single End PCR Primer 1 (97% over 35bp)
ATATACGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAG11310.13516016658998428Illumina Single End PCR Primer 1 (97% over 35bp)
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10490.1253607557496848No Hit
CGTATATACGTATATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG10090.12058055533978261Illumina Single End PCR Primer 1 (100% over 32bp)
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9640.11520282987864267No Hit
ATATACGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTAT9640.11520282987864267Illumina Single End PCR Primer 1 (97% over 35bp)
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9540.11400777977616712No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAA9390.11221520462245381No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9350.11173718458146358No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9070.10839104429453204No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTAAA8950.1069569841715614No Hit
ATACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTAT8890.10623995411007607Illumina Single End PCR Primer 1 (97% over 35bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8860.10588143907933341No Hit
CGTATATATACGTATATATACGTATATATACGAGATCGGAAGAGCGTCGT8580.10253529879240186No Hit
ACATATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTAT8420.100623218628441Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)