FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001619335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001619335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596876
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37800.6332973682976029No Hit
GTATACGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCG28460.47681595507274543TruSeq Adapter, Index 1 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25120.4208579336411583No Hit
ATACGTATACGTATACGTATAGATCGGAAGAGCACACGTCTGAACTCCAG15270.2558320321138729Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTATACGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGAT13290.22265931282209372TruSeq Adapter, Index 1 (97% over 36bp)
ATACGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGAT10370.1737379288160355TruSeq Adapter, Index 1 (97% over 36bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT8020.13436626703033797No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT7710.12917255845435233No Hit
TATACGTATACGTATACGTATAAGATCGGAAGAGCACACGTCTGAACTCC7570.12682701264584267Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT7420.12431392785101093No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6510.10906788009569827No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)