FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001619336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001619336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596876
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT72651.217170735630181No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55020.9217995027442886No Hit
GTATACGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCT28740.4815070466897647Illumina Single End PCR Primer 1 (97% over 35bp)
ATACGTATACGTATACGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG16480.2761042494588491Illumina Single End PCR Primer 1 (100% over 30bp)
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT16390.27459639858195006No Hit
ATACGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTAT13330.2233294687673822Illumina Single End PCR Primer 1 (97% over 35bp)
GTATACGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTAT10460.17524577969293453Illumina Single End PCR Primer 1 (97% over 35bp)
TATACGTATACGTATACGTATAAGATCGGAAGAGCGTCGTGTAGGGAAAG9180.15380078944370357Illumina Single End PCR Primer 1 (100% over 28bp)
CGTATACGTATACGTATACGTATACGAGATCGGAAGAGCGTCGTGTAGGG6860.11493174461697238Illumina Single End PCR Primer 1 (100% over 24bp)
CGTATACGTATACGTATACGTATACGTATACGTATACGTATACGTATACG6510.10906788009569827No Hit
CGTATACGTATACGTATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG6280.10521448341028958Illumina Single End PCR Primer 1 (100% over 30bp)
ATACGTATACGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGG6160.10320401557442416Illumina Single End PCR Primer 1 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)