FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001619337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001619337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153760
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCC178941.5509291360421578Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT81270.7043925946470669TruSeq Adapter, Index 27 (97% over 39bp)
ATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT71390.6187595340452088TruSeq Adapter, Index 27 (97% over 39bp)
ACGTATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT42160.36541395090833445TruSeq Adapter, Index 27 (97% over 39bp)
ATATATACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT40110.3476459575648315TruSeq Adapter, Index 27 (97% over 39bp)
GTATACATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCG34480.29884898072389404TruSeq Adapter, Index 27 (97% over 39bp)
ATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCG34210.2965088059908473TruSeq Adapter, Index 27 (97% over 39bp)
GTATATATACGTATATATACAGATCGGAAGAGCACACGTCTGAACTCCAG25000.21668284565247536Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24820.21512272916377756No Hit
ATGTATACATATGTATACATAGATCGGAAGAGCACACGTCTGAACTCCAG22880.19830814034114547Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT20020.17351962279850228TruSeq Adapter, Index 27 (97% over 39bp)
ATATACGTATATATACGTATATAGATCGGAAGAGCACACGTCTGAACTCC19890.17239287200110942Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTATACATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT18800.16294549993066149TruSeq Adapter, Index 27 (97% over 39bp)
ATACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT15680.13590348079323258TruSeq Adapter, Index 27 (97% over 39bp)
CATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAC15330.1328699209540979TruSeq Adapter, Index 1 (97% over 38bp)
ATATACGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT15300.13260990153931493TruSeq Adapter, Index 27 (97% over 39bp)
GTATACATATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAC14370.12454929968104284TruSeq Adapter, Index 1 (97% over 38bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT13280.11510192761059491No Hit
ACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCG12010.10409443905144916TruSeq Adapter, Index 27 (97% over 39bp)
ATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTA11870.10288101511579531TruSeq Adapter, Index 1 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)