FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001619338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001619338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153760
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG187441.6246013035639995Illumina Single End PCR Primer 1 (100% over 28bp)
ATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTA73510.6371342393565387Illumina Single End PCR Primer 1 (96% over 33bp)
ACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTA66610.5773297739564553Illumina Single End PCR Primer 1 (96% over 33bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT60420.5236791013729025No Hit
ATATATACGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTA37860.32814450145610874Illumina Single End PCR Primer 1 (96% over 33bp)
ACGTATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTA37410.32424421023436417Illumina Single End PCR Primer 1 (96% over 33bp)
GTATACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACG32350.28038760227430315Illumina Single End PCR Primer 1 (96% over 33bp)
ATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACG32290.27986756344473723Illumina Single End PCR Primer 1 (96% over 33bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30840.26729995839689363No Hit
ATGTATACATATGTATACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG26900.23315074192206353Illumina Single End PCR Primer 1 (100% over 30bp)
GTATATATACGTATATATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAG25260.2189363472472611Illumina Single End PCR Primer 1 (100% over 30bp)
ATATACGTATATATACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAG21950.19024753848287337Illumina Single End PCR Primer 1 (100% over 28bp)
GTATACATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTA18990.1645922895576203Illumina Single End PCR Primer 1 (96% over 33bp)
ATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTA16980.14717098876716128Illumina Single End PCR Primer 1 (96% over 33bp)
ATATACGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTA14850.1287096103175704Illumina Single End PCR Primer 1 (96% over 33bp)
ATACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTA14660.12706282069061156Illumina Single End PCR Primer 1 (96% over 33bp)
GTATACATATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGT13740.11908889197060046Illumina Single End PCR Primer 1 (96% over 33bp)
CATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGT13200.11440854250450701Illumina Single End PCR Primer 1 (96% over 33bp)
ATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAG11670.10114755235057551Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)