FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001619340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001619340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1296794
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT228691.7635029156519848No Hit
ATATACGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAG160611.23851590923462Illumina Single End PCR Primer 1 (96% over 33bp)
ACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAG158221.220085842469968Illumina Single End PCR Primer 1 (96% over 33bp)
ATACGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAG133971.0330862110713035Illumina Single End PCR Primer 1 (96% over 33bp)
ATATACGTATATACGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG100000.7711325006130503Illumina Single End PCR Primer 1 (100% over 32bp)
ATACGTATATACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG98830.7621102503558776Illumina Single End PCR Primer 1 (100% over 32bp)
ATATACGTATATACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG92870.7161507533193399Illumina Single End PCR Primer 1 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA88350.68129556429163No Hit
ATATACGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATT83060.6405026550091996Illumina Single End PCR Primer 1 (96% over 33bp)
ACGTATATACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT81970.6320973107525173Illumina Single End PCR Primer 1 (96% over 33bp)
ATACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATT79980.6167517739903177Illumina Single End PCR Primer 1 (96% over 33bp)
ATATACGTATATACGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT76750.5918441942205162Illumina Single End PCR Primer 1 (96% over 33bp)
ATATACGTATATACGTATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTT64620.49830582189615313No Hit
CGTATATACGTATATACGTATATACGAGATCGGAAGAGCGTCGTGTAGGG61550.4746320541273325Illumina Single End PCR Primer 1 (100% over 24bp)
ATATACGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTA55410.42728451858969113Illumina Single End PCR Primer 1 (96% over 33bp)
TACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTA52230.4027625050701962Illumina Single End PCR Primer 1 (96% over 33bp)
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT45280.3491687962775892No Hit
ATATACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGA43730.3372162425180869Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATATACGTATATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG43280.33374614626532817Illumina Single End PCR Primer 1 (100% over 32bp)
ATACGTATATACGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT34940.2694336957141998Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATATACGTATATACGTATATACGTATATACGAGATCGGAAGAGCGTC33710.2599487659566593No Hit
ATATACGTATATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAA25940.20003177065902525Illumina Single End PCR Primer 1 (96% over 33bp)
GTATATACGTATATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT24240.18692251814860342Illumina Single End PCR Primer 1 (96% over 33bp)
ATATACGTATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGAT23050.17774604139130812Illumina Single End PCR Primer 1 (96% over 33bp)
ACATATATGTACATATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAG22420.17288790663744588Illumina Single End PCR Primer 1 (100% over 30bp)
TATACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGAT21770.16787554538346106Illumina Single End PCR Primer 1 (96% over 33bp)
GTATATACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAA21730.16756709238321582Illumina Single End PCR Primer 1 (96% over 33bp)
GTATATACGTATATACGTATATACAGATCGGAAGAGCGTCGTGTAGGGAA19880.1533011411218744Illumina Single End PCR Primer 1 (100% over 26bp)
CGTATATACGTATATACGTATATACGTATATACGTATATACGTATATACG19840.1529926881216292No Hit
ATATTCGTATATACGTATATACGTATATACGTATATACGTATATACGTAT18060.1392665296107169No Hit
ATACGTATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTA14950.11528430884165101Illumina Single End PCR Primer 1 (96% over 33bp)
TATACGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTA14210.10957792833711445Illumina Single End PCR Primer 1 (96% over 33bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA13670.105413812833804No Hit
TACGTATATACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT13000.10024722507969654Illumina Single End PCR Primer 1 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)