FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001619349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001619349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1685158
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCC148060.8786119758503358Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAG82310.48844084649629294TruSeq Adapter, Index 13 (97% over 38bp)
ATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAG73990.4390686214586406TruSeq Adapter, Index 13 (97% over 38bp)
ATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAA36500.21659690070604656TruSeq Adapter, Index 13 (97% over 38bp)
GTATACATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAA36380.215884801306465TruSeq Adapter, Index 13 (97% over 38bp)
ATGTATACATATGTATACATAGATCGGAAGAGCACACGTCTGAACTCCAG23040.1367230847196524Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAG21790.1293053826406782TruSeq Adapter, Index 13 (97% over 38bp)
GTATACATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAG20410.1211162395454907TruSeq Adapter, Index 13 (97% over 38bp)
ATATATACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAG17640.10467861173848386TruSeq Adapter, Index 13 (97% over 38bp)
ACGTATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAG16940.10052469857425833TruSeq Adapter, Index 13 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)