FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001619350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001619350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1685158
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG157420.9341557290176945Illumina Single End PCR Primer 1 (100% over 28bp)
ATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATT81950.4863045482975483Illumina Single End PCR Primer 1 (96% over 33bp)
ACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATT74670.4431038513896026Illumina Single End PCR Primer 1 (96% over 33bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT42710.25344804463439036No Hit
GTATACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAG36880.21885188213805473Illumina Single End PCR Primer 1 (96% over 33bp)
ATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAG36850.21867385728815936Illumina Single End PCR Primer 1 (96% over 33bp)
ATGTATACATATGTATACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG27940.16580047686923124Illumina Single End PCR Primer 1 (100% over 30bp)
GTATACATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATT22200.13173838892258175Illumina Single End PCR Primer 1 (96% over 33bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20940.12426134522697575No Hit
ATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATT20460.12141294762864965Illumina Single End PCR Primer 1 (96% over 33bp)
ACGTATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATT17830.10580610245448795Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)