FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001619353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001619353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences725895
Sequences flagged as poor quality0
Sequence length101
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATATATGTACATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAG121891.6791684747794102Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80811.1132464061606706No Hit
ATATGTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGT64280.8855275211979694TruSeq Adapter, Index 7 (97% over 35bp)
GTACATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGT61270.8440614689452332TruSeq Adapter, Index 7 (97% over 35bp)
ATACATATGTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCC51050.7032697566452448Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATGTACATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTC48940.6742021917770477TruSeq Adapter, Index 7 (97% over 35bp)
ATGTACATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTC45730.6299809201055249TruSeq Adapter, Index 7 (97% over 35bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT42690.5881015849399707No Hit
ATATGTACATATATGTACATATAGATCGGAAGAGCACACGTCTGAACTCC34970.48175011537481316Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATGTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTC33110.45612657478009905TruSeq Adapter, Index 7 (97% over 35bp)
GTACATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTC29900.4119053031085763TruSeq Adapter, Index 7 (97% over 35bp)
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT27080.3730567093036872No Hit
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23590.3249781304458634No Hit
CATATATGTACATATATGTACATATATGAGATCGGAAGAGCACACGTCTG22770.3136817308288389Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22520.3102377065553558No Hit
ACATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTC22180.3055538335434188TruSeq Adapter, Index 7 (97% over 35bp)
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20080.27662402964616095No Hit
CGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19530.26904717624449814No Hit
CGTATATACGTATATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC18980.2614703228428354Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CATATATGTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATT16050.22110635835761372TruSeq Adapter, Index 7 (97% over 35bp)
AGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15650.21559591952004079No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15420.21242741718843633No Hit
GTACATATATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATT13520.18625283270996495TruSeq Adapter, Index 7 (97% over 35bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11720.16145585794088677No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10430.14368469268971407No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10270.1414805171546849No Hit
GCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9690.13349038084020415No Hit
ATGTACATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGT9540.1314239662761143TruSeq Adapter, Index 7 (97% over 35bp)
ATGTACATATATGTACATAGATCGGAAGAGCACACGTCTGAACTCCAGTC9410.1296330736539031Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CATATATGTACATATATGTACATATATGTACATATATGAGATCGGAAGAG8850.12191845928130102No Hit
CGTATATACGTATATACGTATATACGAGATCGGAAGAGCACACGTCTGAA8730.12026532763002915Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT8380.11544369364715282No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8310.11447936685057755No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8310.11447936685057755No Hit
ACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGT7560.10414729403012833TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)