FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001619354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001619354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences725895
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT237353.2697566452448354No Hit
ACATATATGTACATATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAG124221.7112667810082725Illumina Single End PCR Primer 1 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100091.3788495581316857No Hit
GTACATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAG66200.9119776276183194Illumina Single End PCR Primer 1 (96% over 33bp)
ATATGTACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAG62630.8627969609929811Illumina Single End PCR Primer 1 (96% over 33bp)
ATACATATGTATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG54140.7458378966654957Illumina Single End PCR Primer 1 (100% over 28bp)
ATGTACATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATT50250.6922488789700989Illumina Single End PCR Primer 1 (96% over 33bp)
ATATGTACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATT46350.6385221003037629Illumina Single End PCR Primer 1 (96% over 33bp)
ATATGTACATATATGTACATATAGATCGGAAGAGCGTCGTGTAGGGAAAG45080.6210264569944689Illumina Single End PCR Primer 1 (100% over 28bp)
GTACATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATT33620.46315238429800454Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATGTACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATT30160.4154870883529987Illumina Single End PCR Primer 1 (96% over 33bp)
CATATATGTACATATATGTACATATATGAGATCGGAAGAGCGTCGTGTAG24740.3408206421038856Illumina Single End PCR Primer 1 (100% over 22bp)
ACATATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATT22680.312441882090385Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATATACGTATATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG19100.2631234544941073Illumina Single End PCR Primer 1 (100% over 32bp)
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT18160.2501739232258109No Hit
GTACATATATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGAT16170.22275949000888556Illumina Single End PCR Primer 1 (96% over 33bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14330.19741147135605014No Hit
CATATATGTACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGAT13830.19052342280908396Illumina Single End PCR Primer 1 (96% over 33bp)
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13800.190110139896266No Hit
ATGTACATATATGTACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG12170.1676551016331563Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATATACGTATATACGTATATACGAGATCGGAAGAGCGTCGTGTAGGG11750.16186914085370474Illumina Single End PCR Primer 1 (100% over 24bp)
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11240.15484333133579928No Hit
CATATATGTACATATATGTACATATATGTACATATATGAGATCGGAAGAG10190.14037842938717032No Hit
ATGTACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAG9910.1365211222008693Illumina Single End PCR Primer 1 (96% over 33bp)
AAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8760.12067861054284712No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAA8590.11833667403687861No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTAAA7900.1088311670420653No Hit
CGTATATACGTATATACGTATATACGTATATACGAGATCGGAAGAGCGTC7830.10786684024549004No Hit
ATATACGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAG7770.10704027441985411Illumina Single End PCR Primer 1 (96% over 33bp)
GTACATATATGTACATATATGTACAGATCGGAAGAGCGTCGTGTAGGGAA7520.10359625014637103Illumina Single End PCR Primer 1 (100% over 26bp)
AAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7430.10235640140791713No Hit
ATATACGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATT7360.10139207461134186Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)