FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001619471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001619471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320128
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCC392832.97569629611674Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT83670.6338021767586174TruSeq Adapter, Index 27 (97% over 39bp)
ATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT74680.5657027197362681TruSeq Adapter, Index 27 (97% over 39bp)
ATGTATACATATGTATACATAGATCGGAAGAGCACACGTCTGAACTCCAG38590.2923201386532215Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTA32180.24376424104329275TruSeq Adapter, Index 1 (97% over 37bp)
ATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT24590.18626981626024142TruSeq Adapter, Index 27 (97% over 39bp)
GTATACATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT22710.17202877296746982TruSeq Adapter, Index 27 (97% over 39bp)
ATATGTATACATATGTATACATATAGATCGGAAGAGCACACGTCTGAACT20410.1546062200029088Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAC16470.12476062927231299TruSeq Adapter, Index 1 (97% over 38bp)
GTATACATATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAC13600.10302031318175209TruSeq Adapter, Index 1 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)