FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001619472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001619472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320128
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG254731.9295856159402724Illumina Single End PCR Primer 1 (100% over 28bp)
ATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAG55460.4201107771367625Illumina Single End PCR Primer 1 (97% over 35bp)
ACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAG48720.3690551219275707Illumina Single End PCR Primer 1 (97% over 35bp)
ATGTATACATATGTATACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG26510.20081386047413585Illumina Single End PCR Primer 1 (100% over 30bp)
ATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGAT21310.1614237407281718Illumina Single End PCR Primer 1 (97% over 35bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT20360.15422746885150532No Hit
GTATACATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAG15680.11877636108013769Illumina Single End PCR Primer 1 (97% over 35bp)
ATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAG14840.11241334173655887Illumina Single End PCR Primer 1 (97% over 35bp)
ATATGTATACATATGTATACATATAGATCGGAAGAGCGTCGTGTAGGGAA13870.10506556939933098Illumina Single End PCR Primer 1 (100% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)