FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001619473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001619473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1325300
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCC1120518.454764958877234Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT164191.2388893080811891TruSeq Adapter, Index 27 (97% over 39bp)
ATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT150481.1354410322191202TruSeq Adapter, Index 27 (97% over 39bp)
ATGTATACATATGTATACATAGATCGGAAGAGCACACGTCTGAACTCCAG103130.7781634346940316Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTA64110.4837395306723006TruSeq Adapter, Index 1 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61180.46163132875575347No Hit
ATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT58350.4402776729797027TruSeq Adapter, Index 27 (97% over 39bp)
ATATGTATACATATGTATACATATAGATCGGAAGAGCACACGTCTGAACT55420.4181694710631555Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTATACATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT53060.4003621821474383TruSeq Adapter, Index 27 (97% over 39bp)
CATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAC41150.3104957368143062TruSeq Adapter, Index 1 (97% over 38bp)
GTATACATATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAC35530.2680902437184034TruSeq Adapter, Index 1 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23990.18101561910510827No Hit
CATATGTATACATATGTATACATATGAGATCGGAAGAGCACACGTCTGAA21130.1594355994869086Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACATATGTATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC19360.14608013280012072Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTA17430.13151739228853845TruSeq Adapter, Index 1 (97% over 37bp)
GTATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTA16610.12533011393646723TruSeq Adapter, Index 1 (97% over 37bp)
GTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT15840.11952010865464423TruSeq Adapter, Index 1 (100% over 35bp)
ATACATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT15070.11371010337282124TruSeq Adapter, Index 1 (100% over 35bp)
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT14290.10782464347694862No Hit
ATATGTATACATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAG13640.10292009356372142Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)