FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001619474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001619474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1325300
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG725105.471214064740059Illumina Single End PCR Primer 1 (100% over 28bp)
ATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGC105940.799366181241983Illumina Single End PCR Primer 1 (96% over 33bp)
ACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGC96130.7253452048592772Illumina Single End PCR Primer 1 (96% over 33bp)
ATGTATACATATGTATACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG70830.5344450313136648Illumina Single End PCR Primer 1 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54810.4135667396061269No Hit
ATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGA42040.317211197464725Illumina Single End PCR Primer 1 (96% over 33bp)
ATATGTATACATATGTATACATATAGATCGGAAGAGCGTCGTGTAGGGAA38490.29042480947709953Illumina Single End PCR Primer 1 (100% over 26bp)
GTATACATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGC37460.28265298422998564Illumina Single End PCR Primer 1 (96% over 33bp)
ATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGC34150.2576775069795518Illumina Single End PCR Primer 1 (96% over 33bp)
ATACATATGTATACATCTGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG31190.23534294122085567Illumina Single End PCR Primer 1 (100% over 28bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT26960.20342563947785405No Hit
GTATACATATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAG24720.18652380593073267Illumina Single End PCR Primer 1 (96% over 33bp)
CATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAG22280.16811288010261827Illumina Single End PCR Primer 1 (96% over 33bp)
ATACATATGTATCCATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG16850.1271410246736588Illumina Single End PCR Primer 1 (100% over 28bp)
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT14190.10707009733645213No Hit
ATACATATGTATAAATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG13900.1048819135290123Illumina Single End PCR Primer 1 (100% over 28bp)
CATATGTATACATATGTATACATATGAGATCGGAAGAGCGTCGTGTAGGG13350.10073190975628159Illumina Single End PCR Primer 1 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)