FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001619911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001619911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393079
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCC323258.223537762129242Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATT89242.2702815464575825TruSeq Adapter, Index 7 (97% over 38bp)
ATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATT73591.8721427499306755TruSeq Adapter, Index 7 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56711.442712533612836No Hit
ATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCC43181.0985069159125773TruSeq Adapter, Index 7 (97% over 38bp)
GTATACATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCC42971.0931644783872962TruSeq Adapter, Index 7 (97% over 38bp)
ATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATT24100.6131083064727446TruSeq Adapter, Index 7 (97% over 38bp)
ATGTATACATATGTATACATAGATCGGAAGAGCACACGTCTGAACTCCAG24030.6113274939643176Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACATATATGTACATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAG23350.5940281724538833Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTATACATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATT23050.5863961188463388TruSeq Adapter, Index 7 (97% over 38bp)
CATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT19870.5054963506063667TruSeq Adapter, Index 7 (97% over 38bp)
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19100.485907413013669No Hit
GTATACATATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT18010.45817761823959047TruSeq Adapter, Index 7 (97% over 38bp)
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT16800.4273950020224942No Hit
ATATGGATCCGGCGCGCCGTCGAGATATAGATCGGAAGAGCACACGTCTG16090.40933247515130544Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT15600.3968667875923161No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15110.38440110003332667No Hit
ATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA15040.38262028752489957TruSeq Adapter, Index 7 (97% over 37bp)
GTACATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCC12860.3271606979767426TruSeq Adapter, Index 7 (97% over 38bp)
ATATGTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCC11950.3040101353671908TruSeq Adapter, Index 7 (97% over 38bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10670.2714467066416675No Hit
AGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10520.2676306798378952No Hit
GATCCGGCGCGCCGTCGAGATATAGATCGGAAGAGCACACGTCTGAACTC10270.26127063516494137Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATATGTACATATATGTACATATAGATCGGAAGAGCACACGTCTGAACTCC10140.25796341193500544Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT10070.25618259942657834TruSeq Adapter, Index 7 (97% over 38bp)
ATATGGATCCGGCGCGCCCTCGAGATATAGATCGGAAGAGCACACGTCTG9790.24905934939287014Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9370.2383744743423078No Hit
ATATGTATACATATGTATACATATAGATCGGAAGAGCACACGTCTGAACT9210.23430404575161737Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACATATGTATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC9170.23328643860394474Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATACATATGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT9010.22921601001325434TruSeq Adapter, Index 7 (97% over 38bp)
ATATACATGTATATACATGTATATAGATCGGAAGAGCACACGTCTGAACT7960.20250382238684844Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7440.18927492946710459No Hit
CGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7340.18673091159792307No Hit
GTATACATATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAG7300.18571330445025044Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATCTCGAGGGCGCGCCGGATCCATATAGATCGGAAGAGCACACGTCTG7280.18520450087641416Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6870.17477402761276994No Hit
ATGGATCCGGCGCGCCGTCGAGATATAGATCGGAAGAGCACACGTCTGAA6850.17426522403893363Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCCGGCGCGCCGTCGAGATATAGATCGGAAGAGCACACGTCTGAACTCC6800.17299321510434287Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6780.1724844115305066No Hit
ATACATATGTATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAG6700.1704491972351614Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTATATACATGTATATACAGATCGGAAGAGCACACGTCTGAACTCCAGTC6680.16994039366132507Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGAGGGCGCGCCGGATCCATATAGATCGGAAGAGCACACGTCTGAACTCC6440.16383475077528944Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA6430.1635803489883713TruSeq Adapter, Index 7 (97% over 37bp)
ATATGTATACATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAG6320.16078192933227162Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACATATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA6300.16027312575843533TruSeq Adapter, Index 7 (97% over 37bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6040.1536586792985634No Hit
CATATGTATACATATGTATACATATGAGATCGGAAGAGCACACGTCTGAA5770.14678983105177332Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCTCGAGGGCGCGCCGGATCCATATAGATCGGAAGAGCACACGTCTGAA5750.146281027477937Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT5700.14500901854334625No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5610.1427194024610829No Hit
CTCGAGGGCGCGCCGGATCCATATAGATCGGAAGAGCACACGTCTGAACT5480.1394121792311469Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATACATATGTATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAG5290.13457854527970206Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACATATGTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAG5220.132797732771275Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATGTATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTC5220.132797732771275TruSeq Adapter, Index 7 (97% over 38bp)
GTATACATATGTATACATATAGATCGGAAGAGCACACGTCTGAACTCCAG5170.13152572383668423Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATGGATCCGGCGCGCCCTCGAGATATAGATCGGAAGAGCACACGTCTGAA4820.12262166129454893Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTATACATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTC4540.11549841126084069TruSeq Adapter, Index 7 (97% over 38bp)
ATATGTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTC4490.11422640232624993Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4470.11371759875241363No Hit
ATACATATGTACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAG4390.11168238445706843Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTC4390.11168238445706843TruSeq Adapter, Index 7 (97% over 38bp)
GGATCCGGCGCGCCGTCGAGATATAGATCGGAAGAGCACACGTCTGAACT4360.11091917909631396Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATGTACATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATT4080.10379592906260572TruSeq Adapter, Index 7 (97% over 38bp)
CATATGTATACATATGTATACATATGTATACATATGAGATCGGAAGAGCA4080.10379592906260572No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT3960.10074310761958793No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)