FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001619912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001619912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393079
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG335928.54586482615454Illumina Single End PCR Primer 1 (100% over 28bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT120183.0574006751823424No Hit
ATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTA81302.0682865276445703Illumina Single End PCR Primer 1 (96% over 33bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA77411.9693242325334093No Hit
ACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTA68521.7431610439631726Illumina Single End PCR Primer 1 (96% over 33bp)
GTATACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACG40991.0427929245775023Illumina Single End PCR Primer 1 (96% over 33bp)
ATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACG40611.0331256566746125Illumina Single End PCR Primer 1 (96% over 33bp)
ATGTATACATATGTATACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG27640.7031665390417702Illumina Single End PCR Primer 1 (100% over 30bp)
ACATATATGTACATATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAG23540.5988618064053282Illumina Single End PCR Primer 1 (100% over 30bp)
GTATACATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTA22370.5690967973359046Illumina Single End PCR Primer 1 (96% over 33bp)
ATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTA21150.53805977933189Illumina Single End PCR Primer 1 (96% over 33bp)
GTATACATATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGT17580.44723834140211005Illumina Single End PCR Primer 1 (96% over 33bp)
CATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGT16460.4187453412672771Illumina Single End PCR Primer 1 (96% over 33bp)
ATATCTCGACGGCGCGCCGGATCCATATAGATCGGAAGAGCGTCGTGTAG15870.40373563583910616Illumina Single End PCR Primer 1 (100% over 22bp)
ATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAG14940.38007626965571806Illumina Single End PCR Primer 1 (96% over 33bp)
ATATGTACATATATGTACATATAGATCGGAAGAGCGTCGTGTAGGGAAAG12380.3149494122046713Illumina Single End PCR Primer 1 (100% over 28bp)
ATATGTACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACG11910.3029925282195182Illumina Single End PCR Primer 1 (96% over 33bp)
GTACATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACG11170.284166795987575Illumina Single End PCR Primer 1 (96% over 33bp)
ATATGTATACATATGTATACATATAGATCGGAAGAGCGTCGTGTAGGGAA10800.2747539298716034Illumina Single End PCR Primer 1 (100% over 26bp)
ATATCTCGACGGCGCGCCGGATCAGATCGGAAGAGCGTCGTGTAGGGAAA9860.2508401619012972Illumina Single End PCR Primer 1 (100% over 27bp)
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT9800.2493137511797883No Hit
ATATCTCGAGGGCGCGCCGGATCCATATAGATCGGAAGAGCGTCGTGTAG9770.24855054581903382Illumina Single End PCR Primer 1 (100% over 22bp)
ATACATATGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGT9420.23964648327689853Illumina Single End PCR Primer 1 (96% over 33bp)
ACATATGTATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG9130.23226883145627217Illumina Single End PCR Primer 1 (100% over 32bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9050.2302336171609269No Hit
ATATACATGTATATACATGTATATAGATCGGAAGAGCGTCGTGTAGGGAA8690.2210751528318735Illumina Single End PCR Primer 1 (100% over 26bp)
TACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGT8360.21267989386357453Illumina Single End PCR Primer 1 (96% over 33bp)
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7410.18851172410635011No Hit
ATATGGATCCGGCGCGCCCTCGAGATATAGATCGGAAGAGCGTCGTGTAG7190.1829148847941508Illumina Single End PCR Primer 1 (100% over 22bp)
ATACATATGTATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAG6930.17630043833427886Illumina Single End PCR Primer 1 (100% over 30bp)
ATACATATGTATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTT6790.17273881331742474No Hit
ATATCTCGACGGCGCGCCGGATCCATAGATCGGAAGAGCGTCGTGTAGGG6650.16917718830057063Illumina Single End PCR Primer 1 (100% over 24bp)
GTATATACATGTATATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG6650.16917718830057063Illumina Single End PCR Primer 1 (100% over 32bp)
ATATCTCGACGGCGCGCCGGATAGATCGGAAGAGCGTCGTGTAGGGAAAG6620.1684139829398162Illumina Single End PCR Primer 1 (100% over 28bp)
GTATACATATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG6450.1640891525622076Illumina Single End PCR Primer 1 (100% over 30bp)
CATATGTATACATATGTATACATATGAGATCGGAAGAGCGTCGTGTAGGG6110.15543949180699046Illumina Single End PCR Primer 1 (100% over 24bp)
ATATGGATCCGGCGCGCCCTCGAGATCGGAAGAGCGTCGTGTAGGGAAAG6050.15391308108548155Illumina Single End PCR Primer 1 (100% over 28bp)
GTATACATATGTATACATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG5990.15238667036397263Illumina Single End PCR Primer 1 (100% over 30bp)
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5990.15238667036397263No Hit
ACATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAG5700.14500901854334625Illumina Single End PCR Primer 1 (96% over 33bp)
GTATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAG5640.14348260782183733Illumina Single End PCR Primer 1 (96% over 33bp)
ATATGGATCCGGCGCGCCCTCGAGATAGATCGGAAGAGCGTCGTGTAGGG5540.14093858995265582Illumina Single End PCR Primer 1 (100% over 24bp)
AAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5490.13966658101806506No Hit
ATATGGATCCGGCGCGCCCTCGAGAGATCGGAAGAGCGTCGTGTAGGGAA5200.13228892919743868Illumina Single End PCR Primer 1 (100% over 26bp)
ACATATGTATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG5090.129490509541339Illumina Single End PCR Primer 1 (100% over 30bp)
GTATACATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTAC5010.1274552952459938Illumina Single End PCR Primer 1 (96% over 33bp)
ATACATATGTATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAG4950.12592888452448492Illumina Single End PCR Primer 1 (100% over 30bp)
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4930.1254200809506486No Hit
ATATGTATACATATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAG4830.12287606308146709Illumina Single End PCR Primer 1 (100% over 30bp)
CATATGTATACATATGTATACATATGTATACATATGAGATCGGAAGAGCG4760.12109525057304002No Hit
ATATCTCGAGGGCGCGCCGGATCCATAGATCGGAAGAGCGTCGTGTAGGG4700.1195688398515311Illumina Single End PCR Primer 1 (100% over 24bp)
ATACATATGTACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG4560.116007214834677Illumina Single End PCR Primer 1 (100% over 30bp)
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4330.11015597373555952No Hit
TATGTATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTAC4320.10990157194864136Illumina Single End PCR Primer 1 (96% over 33bp)
ATACATATGTATACATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG4260.10837516122713245Illumina Single End PCR Primer 1 (100% over 32bp)
ATATCTCGACGGCGCGCCGGATCCAGATCGGAAGAGCGTCGTGTAGGGAA4220.10735755407945985Illumina Single End PCR Primer 1 (100% over 26bp)
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTAAA4050.10303272370185128No Hit
ATGTACATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTA4000.10176071476726053Illumina Single End PCR Primer 1 (96% over 33bp)
AAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4000.10176071476726053No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)