FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001619913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001619913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1184335
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA318532.6895261898027165No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124311.049618562315561No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT75990.6416258913229788No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT56740.4790874203667037No Hit
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39000.3292987203789468No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34490.2912182786120481No Hit
AGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33120.279650605614121No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31670.2674074480615704No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT27790.23464644716233157No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC27600.23304217134510083Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24520.2070360159920968No Hit
CGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23750.2005344771538458No Hit
CGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22440.18947341757188635No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21650.18280300759497947No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20000.16887113865587017No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17210.1453136148133763No Hit
GGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17030.14379377456547346No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16370.13822102698982974No Hit
AAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15630.13197279485956254No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT15510.13095956802762732No Hit
ATATACATGTATATACATGTATATAGATCGGAAGAGCACACGTCTGAACT14970.12640004728391882Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14660.12378254463475283No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC14090.11896971718306053Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14070.11880084604440466No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13640.11517011656330346No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13590.11474793871666378No Hit
GCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13240.11179269379018605No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13240.11179269379018605No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12960.10942849784900387No Hit
AAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12850.10849970658639659No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12230.1032647012880646No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG12080.10199816774814557TruSeq Adapter, Index 2 (100% over 35bp)
AAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12030.1015759899015059No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)